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Analysing Gene Expression - A Handbook of Methods Possibilities and Pitfalls
Analysing Gene Expression - A Handbook of Methods Possibilities and Pitfalls
Date: 22 April 2011, 12:16

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In the past century, humankind has made truly remarkable progress in understanding the mystery of life, from the discovery of DNA as a basic form of genetic material, the revelation of the chemical and structural nature of genes, and the establishment of a genetic central dogma, to the complete sequencing of the human genome. Although the central genetic dogma applied to a majority of life forms is defined as genetic information flow from DNA, to messenger RNA, then to protein, the complete sequencing of the three billion-letter human genome has shed little light on how precisely such unidirectional information flow of tens of thousands of genes is programmed. If the achievement of complete sequencing of the one-dimensional linear genetic code of the human genome can be compared with man landing on the moon, then the complexity and difficulty in interpreting the genomic instruction into the form of gene expression in a four-dimensional biological context, such as during development and disease, will prove to be a much more challenging and daunting task than that of getting man back from the moon to the earth.

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